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FDA oversight of large-scale sterile drug compounding strengthened and aimed to enhance product safety and quality

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Departamento de Bioqumica y Biologa Molecular, Universidad Peruana Cayetano Heredia

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The resulting cell lysate was filtered through a 0.2-m filter and loaded onto a 5-mL HisTrap HP column (GE Healthcare/Cytiva) equilibrated in wash buffer (50 mM Tris-HCl pH 8.0, 500 mM NaCl, 1 mM TCEP, 25 mM imidazole)