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This research did not involve human participants and/or animals

When the cells reached approximately 80% confluency, cells were harvested with trypsin and seeded at a density of 2.9 10 5 cells per well in 2.5 ml of culture medium into 6 well flat-bottomed plates (Costar, Corning, USA) and incubated for 24 hr

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The RT2 mix was added to the RT1 mixture (final volume: 25 L), and the reaction was carried out in a thermal cycler: 90 min at 37 C, followed by 5 min at 95 C, and held at 4 C

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10.1111/j.1582-4934.2007.00089.x J