The results led the authors to the conclusion that a focus on early changes in smooth muscle might be important in understanding the subsequent development of childhood asthma
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Rabbit polyclonal anti-cleaved caspase-3 antibody (AB3623) was obtained from Merck Millipore (Darmstadt, Germany), and rabbit polyclonal anti-GFAP antibody (ab7260) was bought from Abcam (Cambridge, UK)
Crucially, these cells preserve plasma membrane integrity and nuclear architecture, lacking characteristic apoptotic features such as chromatin margination, plasma membrane blebbing, or apoptotic body formation (Yagoda et al., 2007
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