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Weekly Dosing Schedule for the First Month After the first week, a weekly dosing schedule is used for the next month

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Subsequently, the culture was maintained for another 2 days in an initial-induced medium, which consisted of complete media supplemented with 0.5 mM 3-isobutyl-1-methylxanthine (IBMX), 1 mM dexamethasone (DEX), and 167 nM insulin (all induction chemicals purchased from Sigma, MO, USA)