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Activity & rest: Pair regular activity with adequate rest, and avoid overtraining

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Here is the step-by-step process

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Weigh Fmoc-Glu-OAllyl (40.9 g, 100.0 mmol) and HOBT (16.2 g, 110.0 mmol) and dissolve them in 150 mL of DMF, add DIC (20.4 mL, 120.0 mmol) under ice bath for activation for 5 min, and add the mixture to the reaction In the column, the reaction was carried out at room temperature for 2 hours