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The target itself was validated by a 2014 Nature study showing NNMT knockdown in mouse fat and liver protected against diet-induced obesity by augmenting cellular energy expenditure (Kraus 2014, PMID 24717514) [2]

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10.1371/journal.pmed.0050152 21 KhaderS

The impact of zinc and folic acid supplementation on sperm DNA methylation: results from the folic acid and zinc supplementation randomized clinical trial (FAZST)

With these parameter values, model simulations are performed to collect information on an outcome of interest, which can be the model output or another outcome, and finally a PRCC is calculated for each input factor

doi: 10.1038/s41572-020-0184-y 3 BrandtFThvilumMHegedsLBrixTH