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Materials and methods Cell lines MCF-7 (HTB-22), T47-D (HTB-133), MDA-MB175VII (HTB-25), HCC1428 (CRL-2327), and BT-483 (HTB-121) cell lines were obtained from American Type Culture Collection (ATCC), MFM-223 (ACC 422), Cal-519 (ACC 302), and Cal-120 (ACC 459) cell lines were from Leibniz Institute DSMZ, and Sum149PT (HUMANSUM-0003004), Sum159PT (HUMANSUM-0003006) cell lines were from Bioivit

revealed that the metabolic shift in tumor cells induced by tyrosine kinase inhibitors (TKIs) targeting mesenchymal-epithelial transition factor or epidermal growth factor receptor (EGFR), which results in increased lactate production, prompts CAFs to excessively produce HGF

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Standardized dosing protocols, validated outcome measures, and comprehensive safety monitoring would establish the evidence base necessary for informed clinical decision-making

Medini H, Zirman A, Mishmar D