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Samples were centrifuged at 3,000 g for 10 min at 4C to create a gradient, followed by removal of the top clear layer and middle debris-containing layer (45 ml)

Thysell E, Surowiec I, Hornberg E, Crnalic S, Widmark A, Johansson AI, et al

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Alternatively, a BMI of 27 or higher with at least one weight-related health condition also qualifies you