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The documented protocol starts at 2.5 mg once weekly as a tolerability dose, then titrates up roughly every four weeks 5, 7.5, 10, 12.5, up to a 15 mg maximum maintenance dose

Although the deprotonation of the substrate hydroxyl is recognized as an important step, the significance of the base at the second deprotonation step seems to be underestimated by many research groups

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GHK-Cu often comes in 50 mg vials with daily doses around 1-2 mg