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After 2 days of culture (approximate doubling time of the cells), the cell monolayer was formed, and then the medium was replaced with DMEM without FBS for more than 12 h for serum starvation

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The fusion protein's SASA plot revealed slightly diminishing trends, indicating reduced exposure of the hydrophobic core to solvation as unfolding progressed, making the proteins more stable (Fig

Even with advanced microneedle systems, the delivery of such compounds requires clinical-grade precision and regulation