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Protein extraction employed lysis buffer (2% sodium dodecyl sulfate, 100 mM triethylammonium bicarbonate) with a Covaris ML230 ultrasonicator

However, a significant challenge in evaluating kidney outcomes lies in the inconsistent definitions of renal endpoints across studies, particularly regarding the thresholds for substantial eGFR decline, which vary from 30% to 57%

(2.2 ) To reduce the risk of sustained eGFR decline, end-stage kidney disease and cardiovascular death, increase the dosage to 1 mg once weekly after at least 4 weeks on the 0.5 mg dosage

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