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Researchers and users exploring GHK-Cu for skin regeneration, post-procedural healing, or longer-term tissue repair often stop their protocol prematurely when discomfort becomes a recurring experience

*Correspondence: Giulia Guerriero, Department of Biology, Comparative Endocrinology Lab, Universit degli Studi di Napoli Federico II, Via Mezzocannone, 8, Naples 80134, Italy e-mail: [email protected] This article was submitted to Experimental Endocrinology, a section of the journal Frontiers in Endocrinology

Ice : Applying ice packs to the affected area for 15-20 minutes at a time, several times a day, can help reduce pain and inflammation

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Live/Dead staining results in Supplementary Fig