Reactive Oxygen Species (ROS) are increased by the consumption of sugars and oxidized vegetable oils, smoking, alcohol, exposure to environmental toxins, high or chronic emotional stress, circadian rhythm dysregulation (poor sleep), lack of exercise, infections, and a lack of antioxidant intake
Li D, Zhang Y, Xu L, et al
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Add four times the volume of acetonitrile: methanol (1:1) solution, vortex for 30 s, and sonicate for 5 min on ice
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