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Mass spectrometry was performed on a Q Exactive Orbitrap (Thermo Fisher Scientific) with an Ion Max source and HESI II probe in positive and negative ionization modes (m/z 701000)

The basis of this finding is thought to be the affected cholinergic pathways in the cerebral cortex and basal forebrain in AD [109] and therefore the consequential cholinergic insufficiency adds to the cognitive damage [110]

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It also reduces melanin production, contributing to skin-lightening effects

Understanding these differences helps avoid metabolic adaptation and ensures consistent results

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