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Then pellets were resuspended in lysis buffer (Tris-HCl 100 mM and 0.5% Triton X-100, pH 8) containing Halt Protease and Phosphatase Inhibitor cocktail (Thermo Scientific, 1861280), thoroughly vortexed for 1 min and incubated for 15 min on ice

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Exogenous APN protects normal tissues from radiation-induced oxidative damage and fibrosis in mice and prostate cancer patients with higher levels of APN have less radiation-induced toxicities

Lower the tongue and hold the liquid for 1015 seconds before swallowing

Standard curves for each pair of primers were made, and qPCR results were considered valid only when amplification plots were within the dynamic range and met all other criteria set by the MIQE guidelines (Bustin et al, 2010)

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