Health care companies, such as companies providing medical and healthcare goods and services, companies engaged in manufacturing medical equipment, supplies and pharmaceuticals, as well as operating health care facilities and the provision of managed health care, may be affected by government regulations and government health care programs, increases or decreases in the cost of medical products and services and product liability claims, among other factors
Then, NaCl and PEG 8000 were added into the clarified lysates to reach final concentrations of 200 mM and 10% ( w / v ), respectively, and the mixtures were stirred gently at 4 C overnight to allow protein precipitation
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