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The protein concentration was determined using the BCA Protein Assay Kit (Pioneer, Shanghai, China), and then the protein samples were transferred onto PVDF membranes by SDS-PAGE electrophoresis and then incubated with specific primary antibodies at 4 C overnight

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Scrublet (v.0.2.3) was applied individually for doublet detection in non-multiplexed samples with automatically determined threshold of doublet score 148