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As we age, glutathione production gradually declines: research consistently shows lower cellular glutathione concentrations in older adults compared to younger adults (Detcheverry et al., 2023)

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Additional limitations include variability in study designs, differences in endpoints used across models, and limited evidence on clinically meaningful outcomes beyond biomarker changes

Wang M, Sun J, Xue F, Shang F, Wang Z, Tan T (2012a) The effect of intracellular amino acids on GSH production by high-cell-density cultivation of Saccharomyces cerevisiae

N-Acetylcysteine N-acetylcysteine (NAC) is an antioxidant that scavenges free radicals and promotes liver detoxification