Liver samples were fixed overnight at 4C in 4% formaldehyde, then washed in PBS and continued fixation in 70% ethanol
Yaghubi E, Daneshpazhooh M, DJalali M, Mohammadi H, Sepandar F, Fakhri Z, Ghaedi E, Keshavarz SA, Balighi K, Mahmoudi H, Zarei M, Javanbakht MH (2019) Effects of l -carnitine supplementation on cardiovascular and bone turnover markers in patients with pemphigus vulgaris under corticosteroids treatment: a randomized, doubleblind, controlled trial
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Some report improvements in energy and libido that seem disproportionate to the amount of weight lost, which may reflect these additional mechanisms
The primary commercial enzymes used in GOS synthesis process are bacterial -galactosidases (e.g., Kluyveromyces lactis ) and fungal (e.g., Aspergillus oryzae ) sources (Marz et al., 2022)