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Notably, RT-qPCR analysis showed a sharp increase in OCT4 mRNA expression, a marker of pluripotent stem cells and essential for TIC self-renewal [33], in the colonies formed in 3D gels relative to 2D culture conditions (Fig

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Consistent with the cell viability study, the two compounds with side chains on their 10 nitrogen, chlorpromazine and promethazine, exhibited significantly lower potencies against ROS generation with EC50s of 2148 and 9687 nM, respectively (Figure 4C, Table 1)