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We repeated our GSIS assay in a 2 hour static incubation in INS-1 cells and again did not see any augmentation of GSIS with 100 nM (pGlu-Gln)-CCK-8 treatment (Suppl

Energy transfer occurs when cAMP labeled with HTRF acceptor d2 (cAMP-d2) and the anti-cAMP antibody labeled with europium (Eu) as the energy donor (Anti-cAMP-Cryptate) are in proximity, resulting in the generation of fluorescence signal

Fulurija A, Lutz TA, Sladko K, Osto M, Wielinga PY, Bachmann MF, et al

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doi:10.1073/pnas.0503804102 [87] Bhowmik A, Ojha D, Goswami D, et al