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Supernatants were assayed for total protein concentration with Bradford assay (BioRad 5000006) and 50100 g of protein was mixed with loading buffer and loaded in a 12 or 16% gradient gel (Thermo 1Fisher XP00162)

beyond this, any haze or discoloration warrants immediate disposal

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Coupled steady-state kinetic measurements with GSSR and GSH revealed that both half-reactions usually occur with second order rate constants around 10 5 to 10 6 M 1 s 1 with the reductive half-reaction being slower for wild-type glutaredoxins 11,14,15,17,19

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Disorder in melanin formation has been found to cause a variety of skin diseases in humans such as hyperpigmentation, lentigo, vitiligo and skin cancer 10