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Low-purity or degraded peptide produces poor results regardless of protocol

[9] First, methionine receives an adenosine group from ATP, a reaction catalyzed by S-adenosyl-methionine synthetase, to give S -adenosyl methionine (SAM)

This model is elegant but not fully proven

Without the preservative you lose the multi-withdrawal sterility that reconstitution work depends on.

Lipid-based vehicles, including self-emulsifying systems and liposomal constructs, may enhance solubility or promote lymphatic transport

Sterile water lacks this preservative, making multi-dose vials susceptible to contamination after the first needle insertion