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The size of the molecules selected may permit understanding of the different routes of transport, with molecules such as inulin, polyethylene glycols (PEGs) and Fluorescein Isothiocyanate-Dextran (FITC-Dextran) being utilised for assessment of the paracellular route, whilst larger molecules such as horseradish peroxidase can be utilised for assessment of the transcellular route [21]

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