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This high degree of conservation is an absolute biophysical necessity because this specific domain inserts into the deepest, most highly conserved orthosteric binding pockets of the transmembrane bundles of the GLP-1, GIP, and Glucagon receptors

Transgenes L3781-lag-2p::tig-2::mCherry , L3781-lag-2p::mGreenLantern::hmp-2 , L3781-vha-6p::tig-2::mCherry , L3781-elt-3p::elt-3::mCherry::elt-3 3UTR , and L3781-pqm-1p::pqm-1::mCherry::pqm-1 3UTR were respectively injected into N2 with an injection marker of myo-2p::mCherry , to generate sydEx298 , sydEx390 , sydEx392 , sydEx289 , and sydEx282

Additionally, qPCR analysis demonstrated that TNF induced EAAT3 expression in human lung epithelial cell lines, HBEC and 16HBE (Fig

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[PubMed: 38976257] [Free PMC Article] Dahl D, Onishi Y, Norwood P, et al

2.5.2 cDNA synthesis and RT-qPCR Reverse transcription was conducted with 40 ng of RNA per sample using the Reverse Transcription Master Mix Kit (Fluidigm Corporation, San Francisco, California, USA), following the manufacturers instructions