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In an electrophoretic mobility shift assay (EMSA), GST-tagged proteins are incubated with radiolabeled or fluorescently labeled DNA probes

Upon blocking this system by the synthetic small-molecule erastin the import of cysteine is prevented

It plays a crucial role in various physiological processes such as development, immunity and energy metabolism, and its dysregulation has been found in numerous diseases such as cancer, neurodegenerative disorders and metabolic syndromes

The green labels show the observed 18 O incorporations into the fragments when reactions are performed with H 2 18 O

F., Silva, A