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Drawing through the rubber stopper of a vial dulls the tip, and a dull needle hurts more going through skin

[1] A frequently employed regimen involves applying approximately 1-2 mL of the 0.5 % solution to affected scalp regions once to twice daily, preferably on clean, dry skin in the evening

The characteristic blue colour indicates proper copper(II) complexation

After euthanasia, the knee joints were surgically exposed, and the femoral and tibial condyles were examined for gross morphological changes

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