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Iron overload and transferrin deficiency occur in the follicular fluid of EMs infertility patients (68)

However, HRP isolation and purification gives low yields and recombinant HRP obtained in the yeast Pichia pastoris is often hyper-glycosylated though an unglycosylated, active and stable HRP variant is now available from site-directed mutagenesis (Capone et al., 2013) essentially emphasize the importance of its functional immobilization in nanostructures for improving activity and allowing reusability

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Kuhn, R., Schwenk, F., Aguet, M

PMID: 9226683 Glutathione S-transferase can be used as a C-terminal, enzymatically active dimerization module for a recombinant protease inhibitor, and functionally secreted into the periplasm of Escherichia coli