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Cells were treated with 2 N HCl for 10 min at room temperature to achieve DNA denaturation, followed by a PBS wash and subsequent immunofluorescence staining as previously described

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Over time, this can contribute to: Autoimmune conditions (Hashimotos, lupus, rheumatoid arthritis) Gut disorders (IBS, leaky gut, SIBO, Crohns) Neuroinflammation (brain fog, anxiety, depression, neurodegenerative diseases) Chronic pain syndromes (fibromyalgia, migraines, joint pain) The key to calming this cycle