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Press the purple button and hold the pen for 10 seconds to release all the components of the injections into the desired location

We purchased from the Genome Engineering Facility of the Max Planck Institute of Molecular Cell Biology and Genetics (Dresden, Germany) the body wall muscle driver strain PS6936 ( syIs321 [myo-3p:NLS:GAL4SK:VP64:unc-54 3UTR + myo-2p:NLS:mCherry + pBlueScript] ) that expresses an mCherry reporter in the pharyngeal muscles (44) and the effector strain oxTi10882 [ 15xUAS-T01G9.2a-SL2-mScarlet-glh-2_3UTR ], carrying a transgene inserted in chromosome IV consisting of the myt-1 coding sequence (isoform a) downstream of the UAS element with mScarlet reporter

Cell Metabolism 2022: 10.1016/j.cmet.2022.07.002 receptor binding constants (GIP EC50 0.0643 nM, GLP-1 0.775 nM, glucagon 5.79 nM)

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