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Hydration and stress management count as well

Samples were examined under a Compact Confocal Power Pack (ZEISS LSM 800 with Airyscan) with a 40 (numerical aperture, 1.2) oil immersion lens

The starting resin used for the synthesis was 21.75g (4-((2,4 -dimethoxyphenyl)-(Fmoc-amino)- methyl)-phenoxy resin (Rink resin) with a substitution capacity of 0.55 mmol/g

Proteins were then precipitated by the addition of the total sample volume to 4 volume liquid chromatographymass spectrometry (LCMS)-grade acetonitrile, as per the SP4 protocol 63 , followed by gentle shaking for 5 min and centrifugation at 3,220 g for 10 min

The biotine-spm is removed enzymatically by GspA from the enriched biotin-Gsp- S -thiolated peptides and the GSS-peptides are identified by mass spectrometry

Sendur OF, Turan Y, Tastaban E, Serter M