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Targeted plasma metabolomic analysis For the plasma metabolomic analysis, a 12-h fasted venous blood sample (20 mL) was collected into EDTA-coated vacutainer tubes and centrifuged (Hettich Rotina 46R5) for 15 min at 2,500 RPM at 4 C

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In obesity care, meaningful has a specific clinical definition: losing at least 5% of starting body weighta threshold associated with health improvements

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