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The culture medium consisted of 10% fetal bovine serum, 1% penicillin/streptomycin, 2% Ultroser G (Pall Corp.), and 1% ITS Plus (Zen-Bio) to support optimal cell growth

As we age, our bodies often produce less stomach acid, which is essential for absorbing B12 from our food

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The assay was stopped by cell lysis and the response was measured by adding 20 l/well detection solution (cAMP conjugate and lysis buffer (Cisbio) + 2.5% anti-cAMP cryptate conjugate (Cisbio) + 2.5% cAMP-d2 conjugate (Cisbio)

Prioritize balanced nutrition , including plenty of protein, fiber, iron, and healthy fats

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