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Protein Cell 13 , 689693 (2022)

However, compared with native GLP-1, which is rapidly degraded and has a short half-life of approximately 2 min, semaglutide has been structurally modified by the addition of a hydrophilic spacer and a fatty acid side chain, allowing reversible binding to albumin [36]

Having demonstrated the functional impact of p.D563G and p.S539W on PAM amidation activity in vivo we assessed the impact of this on postprandial amidated, unamidated and total (sum of amidated and unamidated) plasma GLP-1 concentration in humans

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