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The data were, however, not compatible with a protective intention-to-treat effect. They concluded: Randomized trials using MALO as an outcome might be unfeasible, motivating further large observational studies using appropriate methodology to further delineate the effect of GLP1 agonists on the risk of MALO, complementing future phase 3 trials of GLP1 agonists.

doi: 10.1038/nrc3557

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After fixation, the cells were washed twice with phosphate-buffered saline (PBS) to remove excess ethanol and residual treatment compounds