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Statistical analysis Analysis of variance (ANOVA) was conducted using the Statistical Analysis System (SAS version 9.3) (SAS Institute Inc., Cary, NC)

10.1007/s12551-013-0106-2 526 SchulerB.SorannoA.HofmannH.NettelsD

**Quick calculator (30mg vial at 10mg/ml):** | Weekly Dose | Volume per Injection | Syringe Units | Doses per Vial | |------------|---------------------|---------------|----------------| | 1mg | 0.10ml | 10 units | 30 doses | | 2mg | 0.20ml | 20 units | 15 doses | | 4mg | 0.40ml | 40 units | 7.5 doses | | 8mg | 0.80ml | 80 units | 3.75 doses | | 12mg | 1.20ml | Split: 60 units x2 | 2.5 doses | ## Universal Mixing Calculator Use this formula for any vial size and desired concentration: **BAC Water (ml) = Vial Size (mg) Desired Concentration (mg/ml)** **Injection Volume (ml) = Desired Dose (mg) Concentration (mg/ml)** **Syringe Units = Injection Volume (ml) 100** ### Example Calculation You have a 50mg vial and want 10mg/ml concentration: - BAC water: 50mg 10mg/ml = 5.0ml - For a 4mg dose: 4mg 10mg/ml = 0.4ml = 40 units - For an 8mg dose: 8mg 10mg/ml = 0.8ml = 80 units ## Which Vial Size Should You Choose

L-Norvaline was administered at higher (10 mg/kg) and lower (8 mg/kg) dose intraperitoneally to the different groups of diabetic animals along with alpha-tocopherol (900 mg/kg) orally for a period of 30 days