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The assay was performed by heating samples in a Mx3005p qPCR machine (Agilent Technologies, USA) from 25 to 97 C in 1 C min 1 increments for 30 s in an expanding saw-tooth profile, a refinement of a previously described protocol 24

Both the Novo and Lilly pills are less effective than GLP-1 injections

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DMF, via the covalent modification of KEAP1 cysteine residues, leads to reliable NRF2 stabilization, with dose-dependent effects that are cytoprotective at low doses

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