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[DOI] [PMC free article] [PubMed] [Google Scholar] 7.Spera I, Cousin N, Ries M, Kedracka A, Castillo A, Aleandri S, et al

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If a research protocol requires frozen solution storage, the better practice is aliquoting into single-use portions under controlled conditions so each aliquot is thawed once

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On one hand, NADH is overproduced due to influx of hyperglycemia to the glycolytic and Krebs cycle pathways and activation of the polyol pathway