glutathione on kidneys deficiency alters the vitamin D-metabolizing enzymes CYP27B1 and CYP24A1 in human renal proximal tubule epithelial cells and kidney of HFD-fed mice Is Glutathione Safe for Kidneys?

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For flow cytometry analysis, cells were harvested by trypsinization at the indicated time points after release, washed with PBS, and fixed in ice-cold 70% ethanol at 20 C overnight

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Anti-nitrotyrosine-HGF mAbs (3A11C6 anti-nitroY198-HGF and 6B82C3 anti-nitroY250-HGF) were raised in-house against a synthetic peptides FTSNPEVR nitro Y 198 EV and KFLPER nitro Y 250 PDKGFD, respectively (sites well-conserved in mammals) as described previously 32

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