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The gRNAs were expressed using an U6gRNA-Cas9-2A-RFP (Sigma-Aldrich), or pSpCas9 BB-2A-Puro (PX459) v2.0 vector after transient transfection using Lipofectamine LTX following the manufacturers protocol

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Both are generally considered safe for long-term use at standard supplementation levels

Patients often discontinue therapy prematurely or alter dosing behavior to manage costs

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Together with GHK-Cus ability to improve vascular wall repair and reduce oxidative stress, this trio supports nervous system recovery, cognitive function, and peripheral circulation integrity in experimental models