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Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C

DOI: 10.1038/s41398-026-03961-3 To view or add a comment, sign in Im happy to share our latest research: Alcohol-fluoxetine co-treatment drives gut dysbiosis and intestinal barrier disruption with consequences for neuroimmune signaling in male rats. Alcohol use disorder and depression frequently coexist, yet little is known about how alcohol and antidepressant treatment interact through the gut-brain axis

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