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Every 50 oocytes were cultured in 100 l mini-drops of MEM+ containing 20% FBS covered with mineral oil in an incubator at 37.0 C, 5% O 2 , and 5% CO 2 in a humidified atmosphere

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Hangover symptoms were assessed using the Acute Hangover Scale (AHS) and Alcohol Hangover Severity Scale (AHSS) at various intervals post-alcohol consumption

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