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Cells were incubated with 1:100 anti-rabbit GLUT1 (Abcam, ab115730) antibody in BD Stain Buffer (BSA) (BD Biosciences) 30 min at room temperature after 20 min on ice to block nonspecific binding, followed by incubation for 30 min at room temperature with 1:200 Alexa Fluor 647 (Abcam, ab150075)
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It promotes collagen synthesis, reduces inflammation, and enhances wound healing
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These contraindications are treated as standard screening criteria across the growth-factor research literature, not unique cautions specific to a single study
While 2.4 mg is the FDA-approved maintenance dose for semaglutide, not everyone needs the absolute max to achieve substantial weight loss
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