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when the OFEL was hormonally primed (with a mixture of E2 + P4 + cAMP + XAV939) to reach the receptive WOI state, supporting the physiological relevance of this new embryo adhesion model

For each observation, the mica was rinsed after incubation for 3 min and immersed in a liquid cell containing approximately 90 L of imaging buffer (150 mM NaCl and 20 mM 4-[2-hydroxyethyl]-1-piperazineethanesulfonic acid-NaOH [pH 7.5])

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Observing for any adverse effects while undergoing treatment is vital

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