Cell viability and ROS and glutathione intracellular content of cells cultured in liquid medium were assessed by flow cytometry analysis of cells stained for 30 min in the dark with 0.025 M calcein-AM (ThermoFisher Scientific, C3099) at room temperature, 50 M Dichlorodihydrofluorescein-diacetate (DCFDA, ThermoFisher Scientific, D399) at 37 C, and 10 M monochlorobimane (ThermoFisher Scientific, M1381MP) at 37 C, respectively
The thigh, buttocks, or upper arm are the best spots due to their muscle depth and ease of access
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Correlation of anti-Helicobacter pylori cagA IgG antibodies with resistance to first line treatment, bleeding gastroduodenal ulcers and gastric cancer
Reduced effectiveness despite consistent dosing can also indicate degradation, though this is harder to confirm objectively
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