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Tetramethylbenzidine (TMB) substrate was then applied and incubated for 30 min, followed by the addition of the stop solution, and absorbance was read at 450 nm using a microplate reader (IRE 96 SFRI plate reader, France)

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Therefore, from the above observations, we hypothesize that altered cytokines in shECM1-MenSCs could contribute to HSC inactivation

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Bypassing GSSG recycling restores proteostasis Collectively, our data show that G6PDH deletion in growth plate chondrocytes decreases NADPH levels and the GSH:GSSG ratio, resulting in increased ROS levels, altered proteostasis and increased lipid peroxidation and ferroptosis