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Though oxalate can be found in relatively small amounts in plants, it can be accumulated at high levels in several plants species (called extreme oxalate accumulators

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qRTPCR was performed via SYBR Premix Ex Taq II (Takara) on a Chromo 4 continuous fluorescence detector with a PTC-200 DNA Engine Cycler (Bio-Rad Laboratories, Hercules, CA)