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doi: 10.1158/0008-5472.CAN-14-0155

Cells were grown at 30C in Hartwells complete (HC) medium with 2% glucose and lacking leucine and uracil (-Leu-Ura) to ensure retention of both plasmids to an OD 6 00 of 3.0

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PMID: 296 GreenRSRoyTMoralesD-IMorrowCNeilsonRSchottEMet al

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