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Intravital fluorescence signals (excitation at 620 nm, emission at 670 nm) were collected at various time points (1, 3, 5, 8, 10, and 24 h) post-injection using an in vivo fluorescence imager (IVIS Lumina III, PerkinElmer, US)

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Furthermore, RT has a high quantum yield and photostability

For more on TMAO see: Egg Industry Response to Choline & TMAO How to Develop a Healthy Gut Ecosystem How Our Gut Bacteria Can Use Eggs to Accelerate Cancer How to Reduce Your TMAO Levels Can Vegan Fecal Transplants Lower TMAO Levels