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Aggressive shaking introduces mechanical stress and can damage the peptides structure

We found that DRP1 levels increased in NAM-treated and NAM-pre-treated neurons, but not in neurons treated only with Rot (data not shown), probably because of the low NAD + availability resulting from CI inhibition induced by Rot

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It is found abundantly in animal proteins and needed in high concentrations in serum and cerebrospinal fluid

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Using these enzyme preparations with a defined GSH concentration (20 M) in the established two-step in vitro reactions, we found that the extent of conversion of the ultrafiltered flavan-3,3,4-triol ( 4 ) depended on the applied concentration of the purified Pt GSTF8, confirming that this enzyme is required and sufficient for the observed conversion to cyanidin (Extended Data Fig